Inhibition of long non-coding RNA TUG1 on gastric cancer cell transference and invasion through regulating and controlling the expression of miR-144/c-Met axis
10.1016/j.apjtm.2016.03.026
- Author:
Ting-Ting JI
1
;
Xiao-Ju ZHUGE
1
;
Xuan HUANG
2
;
Jie JIN
3
;
Sheng-Hua PAN
4
Author Information
1. Department of Gastroenterology, The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital)
2. Department of Gastroenterology, Zhejiang Provincial Hospital of Chinese Traditional Medicine
3. Department of Gastroenterology, Wenzhou Central Hospital
4. Department of Pathology, The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital)
- Publication Type:Journal Article
- Keywords:
c-Met;
Gastric carcinoma;
Invasion;
lncRNA-TUG1;
microRNA-144;
Transference
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(5):508-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the expression of long noncoding RNA TUG1 (lncRNA-TUG1) in gastric carcinoma (GC) and its effects on the transferring and invading capacity of gastric carcinoma cells. Methods Forty cases of carcinoma tissue and para-carcinoma tissue were selected from GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December, 2014; the expressing level of lncRNA-TUG1 in GC and para-C tissues was detected by applying the qRT-PCR technique. The correlation between lncRNA-TUG1 expression and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected using lncRNA-TUG1 specific siRNA. Changes of the transferring and invading capacity of siRNA-transfected SGC-7901 cells were scratch-tested and transwell-detected. qRT-PCR was applied to detect the expression level of microRNA-144 after lncRNA-TUG1 was silenced. Changes of c-Met mRNA and protein expressions was detected by qRT-PCR and western-blot test. Results The expression level of lncRNA-TUG1 in GC tissue was significant higher than that in para-C tissue (P < 0.05) and the high expression level of lncRNA-TUG1 in GC tissue was significantly correlated with tumor lymph nodes metastasis and advance TNM phasing (P < 0.05). The transferring and invading capacity of SGC-7901 cells was highly inhibited after being transfected by lncRNA-TUG1 specific siRNA (P < 0.05). The results of qRT-PCR and western-blot proved that the expression of microRNA-144 was significantly boosted and the expression level of c-Met mRNA and protein was inhibited after lncRNA-TUG1 was silenced (P < 0.05). Conclusions lncRNA-TUG1 shows an up-regulated expression in GC tissue and that bears a correlation with clinicopathological features of malignant tumor. lncRNA-TUG1 can promote the transferring and invading capacity of GC by inhibiting the pathway of microRNA-144/c-Met.
