Polarization of M1 tumor associated macrophage promoted by the activation of TLR3 signal pathway
10.1016/j.apjtm.2016.03.019
- Author:
Bo LIU
1
;
Xia WANG
1
;
Tai-Zhong CHEN
1
;
Guang-Liang LI
1
;
Chang-Chun TAN
1
;
Yong CHEN
1
;
Shao-Qiang DUAN
1
Author Information
1. Department of Pediatric Surgery, Yongchuan Hospital of Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
M1;
Poly-ICLC;
TLR3;
Tumor-associated macrophage
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(5):484-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation between activation of toll-like receptors 3 (TLR3) signaling pathway and tumor-associated macrophage and its effect on the tumor growth. Methods The mice Lewis lung cancer cell lines 3LL and melanoma B16H10 were used to construct the subcutaneous transplantation tumor models and then they were treated with Poly-ICLC. The curative effect was observed and then the T cell and macrophage phenotypes infiltrated in local tumor were detected by flow cytometry. After the in vitro culture of mouse bone marrow-derived macrophage, the real-time PCR and western blot were applied to detect the expression of macrophage activation markers and the activation of intracellular signaling pathways. Results The survival time of mice with brown tumor treated with Poly-ICLC significantly increased and the tumor growth was inhibited. The ratio of local tumor-infiltrated Treg decreased, while the ratio of CD8
