Zika Virus spreading in South America: Evolutionary analysis of emerging neutralizing resistant Phe279Ser strains
10.1016/j.apjtm.2016.03.028
- Author:
Marta GIOVANETTI
1
;
Laura CARCANGIU
1
;
Eleonora CELLA
1
;
Alessandra LO PRESTI
1
;
Massimo CICCOZZI
1
;
Marta GIOVANETTI
2
;
Marta GIOVANETTI
3
;
Luiz Carlos ALCANTARA
3
;
Teresa MILANO
4
;
Stefano PASCARELLA
4
;
Eleonora CELLA
5
;
Alessia LAI
6
;
Gianguglielmo ZEHENDER
6
;
Silvia ANGELETTI
7
;
Massimo CICCOZZI
8
Author Information
1. Department of Infectious Parasitic and Immunomediated Diseases, National Institute of Health
2. Department of Biology, University of Rome ‘Tor Vergata’
3. Laboratory of Hematology, Genetic and Computational Biology, Gonçalo Moniz Research Center, Oswaldo Cruz Foundation (LHGB/CPqGM/FIOCRUZ)
4. Dipartimento di Scienze Biochimiche ‘A. Rossi Fanelli’, Università La Sapienza
5. Department of Public Health and Infectious Diseases, Sapienza University of Rome
6. Laboratory of Infectious Diseases and Tropical Medicine, University of Milan
7. Clinical Pathology and Microbiology Laboratory, University Hospital Campus Bio-Medico of Rome
8. University Campus Bio-Medico
- Publication Type:Journal Article
- Keywords:
Evolution;
Phylogeny;
Zika Virus
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(5):445-452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genetic diversity of Zika Virus (ZIKV) and the relationships existing among these circulating viruses worldwide. To evaluate the genetic polymorphisms harbored from ZIKV that can have an influence on the virus circulation. Methods Three different ZIKV dataset were built. The first dataset included 63 E gene sequences, the second one 22 NS3 sequences and the third dataset was composed of 108 NS5 gene sequences. Phylogenetic and selective pressure analysis was performed. The edited nucleic acid alignment from the Envelope dataset was used to generate a conceptual translation to the corresponding peptide sequences through UGene software. Results The phylogeographic reconstruction was able to discriminate unambiguously that the Brazilian strains are belonged to the Asian lineage. The structural analysis reveals instead the presence of the Ser residue in the Brazilian sequences (however already observed in other previously reported ZIKV infections) that could suggest the presence of a neutralization-resistant population of viruses. Conclusions Phylogenetic, evolutionary and selective pressure analysis contributed to improve the knowledge on the circulation of ZIKV.
