The inhibition effect of Chlorpromazine against the β-lactam resistance of MRSA
10.1016/j.apjtm.2016.04.008
- Author:
Ryong KONG
1
;
Su-Hyun MUN
1
;
Ok-Hwa KANG
2
;
Yun-Soo SEO
2
;
Tian ZHOU
2
;
Dong-Yeul KWON
2
;
Dong-Won SHIN
3
Author Information
1. BK21 Plus Team, Professional Graduate School of Oriental Medicine, Wonkwang University
2. Department of Oriental Pharmacy, College of Pharmacy, Wonkwang Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University
3. Department of Oriental Medicine Resources, College of Bio Industry Science, Sunchon National University
- Publication Type:Journal Article
- Keywords:
blaZ;
Chlorpromazine;
mecA;
MRSA;
PBP2a;
β-Lactamase
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(6):542-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene related to β-lactam resistance and to confirm the mechanism about a synergy effect between CPZ and β-lactam antibiotics. Methods To measure antibacterial activity, we performed a minimum inhibitory concentration (MIC) and synergy test. Transmission electron microscopy (TEM) was used in morphological analysis. To analyze gene expression, we conducted reverse transcriptase polymerase chain reaction (PCR). Results We confirmed a synergy effect between CPZ and β-lactam antibiotics. Furthermore, we observed that CPZ affect the cell envelope of MRSA by using TEM. At the gene level, CPZ reduced the expression of resistance genes. Conclusions Through this result, we hypothesize that a decrease of resistance factor expressions was caused by CPZ because it disrupts the activity of a sensor protein located in the cell membrane.
