Effect of hGC-MSCs from human gastric cancer tissue on cell proliferation, invasion and epithelial-mesenchymal transition in tumor tissue of gastric cancer tumor-bearing mice
10.1016/j.apjtm.2016.06.004
- Author:
Lin SONG
1
;
Xin ZHOU
1
;
Mei DU
1
;
Jin-Ling ZHANG
1
;
Liang LI
1
;
Hong-Jun JIA
2
Author Information
1. Oncology Department No. 2, Linyi People's Hospital of Shandong Province
2. Radiotherapy Technology Department, Linyi People's Hospital of Shandong Province
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transition;
Gastric cancer;
Invasion;
Mesenchymal stem cells;
Proliferation
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(8):796-800
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hGC-MSCs from human gastric cancer tissue on cell proliferation, invasion and epithelial-mesenchymal transition in tumor tissue of gastric cancer tumor-bearing mice. Methods BABL/c nude mice were selected as experimental animals and gastric cancer tumor-bearing mice model were established by subcutaneous injection of gastric cancer cells, randomly divided into different intervention groups. hGC-MSCs group were given different amounts of gastric cancer cells for subcutaneous injection, PBS group was given equal volume of PBS for subcutaneous injection. Then tumor tissue volume were determined, tumor-bearing mice were killed and tumor tissues were collected, mRNA expression of proliferation, invasion, EMT-related molecules were determined. Results 4, 8, 12, 16, 20 d after intervention, tumor tissue volume of hGC-MSCs group were significantly higher than those of PBS group and the more the number of hGC-MSCs, the higher the tumor tissue volume; mRNA contents of Ki-67, PCNA, Bcl-2, MMP-2, MMP-7, MMP-9, MMP-14, N-cadherin, vimentin, Snail and Twist in tumor tissue of hGC-MSCs group were higher than those of PBS group, and mRNA contents of Bax, TIMP1, TIMP2 and E-cadherin were lower than those of PBS group. Conclusion hGC-MSCs from human gastric cancer tissue can promote the tumor growth in gastric cancer tumor-bearing mice, and the molecular mechanism includes promoting cell proliferation, invasion and epithelial-mesenchymal transition.
