Expression of RUNX2 and MDM21 in rats with periodontitis under chronic intermittent hypoxia
10.1016/j.apjtm.2016.06.002
- Author:
Kun LI
1
;
Su-Ge DONG
1
;
Shu ZHOU
1
;
Li MA
1
;
Qiong-Qiong YU
1
;
Zhi-Yong JIANG
1
;
Qiang-Fu HU
2
;
Hua-Xiang ZHANG
3
;
Dan ZHOU
4
Author Information
1. Department of Stomatology, The Fifth Affiliated Hospital of Zhengzhou University
2. Department of Anesthesiology, The Fifth Affiliated Hospital of Zhengzhou University
3. Department of Periodontics, Wuhan University Stomatological Hospital
4. Department of Cardiac Surgery, The Fifth Affiliated Hospital of Zhengzhou University
- Publication Type:Journal Article
- Keywords:
Chronic intermittent hypoxia;
MDM21;
Periodontitis;
RUNX2
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(8):781-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the expression of RUNX2 and MDM21 in rats with periodontitis under the chronic intermittent hypoxia. Methods A total of 32 SD healthy rats were randomly divided into four groups, with 8 rats in each group. The molecular biological techniques of immunohistochemistry, RT-PCR and Western blotting were employed to detect the effect of different hypoxia time (0, 6, 12, 24 and 48 h) and different concentrations of hypoxia (0.000, 0.001, 0.010, 0.060 and 0.100 ppm) on the expression of RUNX2 and MDM21 in rats of four groups. Results The expression of RUNX2 and MDM21 in each group was significantly higher than the one at other concentrations when the concentration was 0.010 ppm, with the statistical difference (P < 0.05). The expression of RUNX2 and MDM21 was that normoxic control group > normoxic periodontitis group > hypoxia control group > hypoxia periodontitis group under the action with the concentration of 0.010 ppm for 12 h, but there was no significant difference for the comparison among groups (P > 0.05). Conclusions The condition of chronic intermittent hypoxia can reduce the expression of RUNX2 and MDM21 in rats with periodontitis and aggravate the damage of periodontal bone.
