Development and application of quantitative detection method for nervous necrosis virus (NNV) isolated from sevenband grouper Hyporthodus septemfasciatus
10.1016/j.apjtm.2016.06.014
- Author:
Jong-Oh KIM
1
;
Jae-Ok KIM
1
;
Wi-Sik KIM
1
;
Myung-Joo OH
1
Author Information
1. Department of Aqualife Medicine, College of Fisheries and Ocean Science, Chonnam National University
- Publication Type:Journal Article
- Keywords:
Diagnostic;
Nervous necrosis virus (NNV);
Quantitative detection;
Sevenband grouper
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(8):742-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the rapid and efficient quantitative detection tool for nervous necrosis virus isolated from sevenband grouper Hyporhodus septemfasciatus. Methods The viral genes of the NNV (SGYeosu08) isolated from sevenband grouper were phylogenetically analyzed. In addition, novel quantitative PCR primers based on the genomic sequence of SGYeosu08 isolate were designed and compared it with the conventional bio-assay method (TCID
