Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain
10.1016/j.apjtm.2016.07.007
- Author:
Áurea Regina Telles PUPULIN
1
;
Lívia BRACHT
2
;
Márcia Machado DE OLIVEIRA DALALIO
3
;
Francielli Maria DE SOUZA SILVA-COMAR
4
;
Bruno Ambrósio DA ROCHA
4
;
Franciele Queiroz AMES
4
;
Roberto Kenji Nakamura CUMAN
4
;
Ciomar Aparecida BERSANI-AMADO
4
Author Information
1. Laboratory of Parasitology, Department of Basic Sciences of Health, State University of Maringá
2. Laboratory of Hepatic Metabolism, Department of Biochemistry, State University of Maringá
3. Laboratory of Immunology, Department of Basic Sciences of Health, State University of Maringá
4. Laboratory of Inflammation, Department of Pharmacology and Therapeutic, State University of Maringá
- Publication Type:Journal Article
- Keywords:
Canova medication;
Cytokines;
Trypanosoma cruzi
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(9):860-865
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain. Methods Animals were divided into five groups: non-treated infected animals (I); benznidazole-treated infected animals (Bz; 100 mg/kg body weight, single daily dose by gavage); Canova medication (CM) treated infected animals (CM; 0.2 mL/animal, single daily dose by gavage); benznidazole- and Canova medication-treated infected animals with the above-mentioned dose (Bz+CM); and non-infected animals (C). TNF-α and IL-10 levels were determined in serum aliquots after 4, 7, 10, 13, and 29 days of infection. An ELISA technique was employed with R&D System Inc. antibody pairs. Results A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10th day after infection, coupled with a IL-10 decrease on the 13th day after infection when compared with the other experimental groups. Conclusions CM may change the balance between plasma cytokine levels (TNF-α and IL-10) in mice infected with Y strain T. cruzi, with important consequences leading towards a more severe infection.
