Effect of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7
10.1016/j.apjtm.2016.07.015
- Author:
Chang-Jiang LEI
1
;
Yuan LI
1
;
Ning LIU
1
;
Chun YAO
2
;
De-Ke LI
3
;
Zhi-Xiong LONG
4
;
Dan TAO
4
;
Yan-Ping LIOU
4
;
Jiang-Zhou ZHANG
4
Author Information
1. Tumor Laboratory, The Fifth Hospital of Wuhan
2. Wuhan Hematology Institute
3. Department of Anesthesiology, The Fifth Hospital of Wuhan
4. Oncology Department, The Fifth Hospital of Wuhan
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Liver cancer;
MiR-132;
miR-520c-3p;
Proliferation
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(9):898-902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of co-transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of hepatocellular carcinoma Huh7. Methods Hepatocellular carcinoma Huh7 was cultured in vitro and lipidosome was used to transfect miR-520c-3p and miR-132, respectively or together. The effects of transfection of miR-520c-3p and miR-132 on proliferation and apoptosis of Huh7 were detected by CCK8 and Annexin V staining and flow cytometry, and the expression level of the targeted gene of over-expressed miR-520c-3p and miR-132 was determined by Western blot and realtime PCR. Results Compared with the control group, the proliferation ability of Huh7 of the single transfected and co-transfected miR-520c-3p and miR-132 decreased significantly, and the apoptosis ratio increased distinctly (P < 0.05). Besides, the effect of the co-transfection group was better than that of the single transfection group. The protein levels of GPC3 (Glypican-3) and YAP (Yes-associated protein), the target genes transfected only by miR-520c-3p and miR-132, respectively, reduced obviously (P < 0.05), which was similar with the co-infected cells, but cells transfected by miR-132 only showed a decrease of YAP. Conclusions The co-transfection of miR-520c-3p and miR-132 can target-regulate the expression of GPC3 and YAP, enhance the exhibition effect on proliferation of hepatocellular carcinoma Huh7 and induce cell apoptosis synergistically.
