Skin whitening and anti-corrugation activities of glycoprotein fractions from liquid extracts of boiled sea cucumber
10.1016/j.apjtm.2016.08.001
- Author:
So Jung KIM
1
;
Sun-Mee HONG
1
;
Eun-Hye KWON
1
;
So Yun PARK
2
;
Taek-Kyun LEE
2
Author Information
1. Gyeongbuk Institute for Marine Bio-Industry
2. South Sea Environment Research Department, Korea Institute of Ocean Science and Technology
- Publication Type:Journal Article
- Keywords:
Cytotoxicity;
Elastase;
Glycoprotein;
Sea cucumber;
Tyrosinase
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(10):1002-1006
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine skin whitening and wrinkle improvement efficacy, glycoprotein fractions were extracted from liquid extracts of boiled sea cucumber and their effects on tyrosine and elastase inhibitory activities were assayed. Methods Fractions above and below 50 kDa (>50 kDa and <50 kDa) were extracted via a series of steps involving: boiling, filtering, desalting and freeze drying. Cytotoxicity, skin whitening and wrinkle-removing effects of boiled liquid were determined. Results Our MTT data showed that neither glycoprotein fraction of boiled liquid induces cellular cytotoxicity up to a concentration of 10 mg/mL treatment of the mouse melanoma cell line, B16F10, with 10 mg/mL >50 kDa enhanced tyrosinase and elastase inhibitory activities by 50.84% and 28.78%, respectively. Correlations of the >50 kDa concentration with tyrosinase inhibitory (R2 = 0.968) and elastase inhibitory (R2 = 0.983) efficacy were significant. Conclusions >50 kDa glycoprotein fraction isolated from liquid extracts of boiled sea cucumber, which can serve as a functional cosmetic ingredient for whitening and wrinkle improvement of skin.
