In vitro biofilm formation in ESBL producing Escherichia coli isolates from cage birds
10.1016/j.apjtm.2016.10.003
- Author:
Ebru Sebnem YILMAZ
1
;
Nur Ceyhan GÜVENSEN
2
Author Information
1. Mustafa Kemal University, Faculty of Art and Sciences, Biology Department
2. Muğla Sıtkı Koçman University, Faculty of Sciences, Biology Department
- Publication Type:Journal Article
- Keywords:
Biofilm formation;
Cage birds;
Escherichia coli;
Extended spectrum beta lactamases;
Hydrophobicity
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(11):1069-1074
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine biofilm and hydrophobicity formation ratios in extended spectrum beta lactamases (ESBL) synthesizing Escherichia coli isolates which were isolated from feces samples of 150 cage bird species randomly taken from pet shops in Hatay province, Turkey. Methods In vitro biofilm production of 4 ESBL positive isolates were performed by Congo Red Agar (CRA), Standard Tube (ST) and Microtitre Plate (MP) methods while their hydrophobicity were examined by bacterial adhesion to hydrocarbon (BATH) test. Results In the examined isolates, while biofilm production was found to be negative by CRA method, highest biofilm producing strain, among 4 bacteria was determined to be A42 by ST and MP methods. The Scanning Electron Microscopy (SEM) also displayed these confirmed findings. The hydrophobicity values of strains were determined to be between 22.45% and 26.42%. Conclusions As a result, biofilm formation in cage bird feces originated ESBL positive Escherichia coli isolates was performed for the first time in Turkey. In order to present the relation between pathogenicity and biofilm production in animal originated ESBL positive isolates, further studies are required.
