Influence of bioactive sulfated polysaccharide-protein complexes on hepatocarcinogenesis, angiogenesis and immunomodulatory activities
10.1016/j.apjtm.2016.11.004
- Author:
Azza A. MATLOUB
1
;
Hadeer A. AGLAN
2
;
Hanaa H. AHMED
2
;
Sahar Salah MOHAMED EL SOUDA
3
;
Mona Elsayed ABOUTABL
4
;
Amany Sayed MAGHRABY
5
;
Amany Sayed MAGHRABY
6
Author Information
1. Pharmacognosy Department, National Research Centre
2. Hormones Department, National Research Centre
3. Chemistry of Natural Compounds Department, National Research Centre
4. Medicinal and Pharmaceutical Chemistry Department (Pharmacology Group), National Research Centre
5. Therapeutical Chemistry Department, National Research Centre
6. Laboratory of Immune and Biomarkers for Infection, The Center of Excellence for Advanced Sciences, National Research Centre
- Publication Type:Journal Article
- Keywords:
Anti-angiogenesis;
Anti-tumor activity;
Enteromorpha intestinalis;
Jania rubens;
Polysaccharide-protein complexes;
Pterocladia capillacea
- From:
Asian Pacific Journal of Tropical Medicine
2016;9(12):1200-1211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the in vivo anticancer, anti-angiogenesis and immunomodulatory efficacies of the bioactive polysaccharide isolated from cold aqueous extract of Jania rubens (JCEM) and Pterocladia capillacea (PCEM) as well as hot aqueous extract of Enteromorpha intestinalis (EHEM) against hepatocellular carcinoma rat model (HCC) and to study their chemical composition. Methods The sugars and amino acids composition of the bioactive polysaccharides of JCEM, PCEM and EHEM were determined using gas liquid chromatography and amino acid analyzer, respectively. These polysaccharide extracts (20 mg/kg b.wt. for 5 weeks) were assessed on hepatocarcinogenesis in rats and α-fetoprotein (AFP), carcinoembryonic antigen (CEA), glypican-3 (GPC-3), hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF) and Ig G levels were evaluated. Results The GLC analysis of JCEM, PCEM and EHEM polysaccharide revealed the presence of 10, 9 and 10 sugars, in addition the amino acid analyzer enable identification of 16, 15 and 15 amino acids, respectively. These polysaccharide extracts of JCEM, PCEM and EHEM produced significant decrease in serum AFP, CEA, GPC-3, HGF and VEGF compared with untreated HCC group. JCEM, PCEM and EHEM had an immunostimulatory responses by increasing the IgG levels as compared by naïve value (1.23, 1.53 and 1.17 folds), respectively. The bioactive polysaccharides in HCC induced rats improved the humoral immune response. The photomicrographs of liver tissue sections of the groups of HCC treated with polysaccharide extracts of Jania rubens and Enteromorpha intestinalis showed intact histological structure. Moreover, fractions HE1, HE4, HE7 obtained from polysaccharide of EHEM showed moderate cytotoxic activity against HepG2 in vitro with IC
