- Author:
Nermine Mogahed Fawzy Hussein MOGAHED
1
;
Safaa Ibrahim KHEDR
1
;
Rasha Abdelmawla GHAZALA
2
;
Inas Mohamed MASOUD
3
Author Information
- Publication Type:Journal Article
- Keywords: Biomarker; microRNAs; miRNA712_3p; Real-time PCR; Toxoplasma gondii
- From: Asian Pacific Journal of Tropical Medicine 2018;11(12):688-692
- CountryChina
- Language:Chinese
- Abstract: Objective: To assess the role of miRNA712_3p as a specific biomarker in early detection of toxoplasmosis in plasma of mice acutely infected with Toxoplasma gondii. Methods: Real-time PCR was used to measure the level of miRNA712_3p in plasma of infected mice. Immune-competent and immune-suppressed mice were examined, three and five days post-infection. Results: Results revealed significant up-regulation of plasma miRNA712_3p in both immune-competent and immune-compromised groups in comparison to the control non-infected group. Additionally, an increase in the level of miRNA712_3p was noticed correspondently in the parasite density detected in liver impression smears. Conclusions: miRNA712_3p can be used as a novel biomarker for the detection of Toxoplasma gondii infection in both immune-competent and immune-compromised host.