Can miRNA712_3p be a promising biomarker for early diagnosis of toxoplasmosis?

Author: Nermine Mogahed Fawzy Hussein MOGAHED ¹ ; Safaa Ibrahim KHEDR ¹ ; Rasha Abdelmawla GHAZALA ² ; Inas Mohamed MASOUD ³
Author Information

1. Medical Parasitology Department, Faculty of Medicine, Alexandria University
2. Medical Biochemistry Department, Faculty of Medicine, Alexandria University
3. Pharmaceutical Chemistry, Faculty of Pharmacy and Drug Manufacturing, Pharos University in Alexandria
Publication Type:Journal Article
Keywords: Biomarker; microRNAs; miRNA712_3p; Real-time PCR; Toxoplasma gondii
From: Asian Pacific Journal of Tropical Medicine 2018;11(12):688-692
CountryChina
Language:Chinese
Abstract: Objective: To assess the role of miRNA712_3p as a specific biomarker in early detection of toxoplasmosis in plasma of mice acutely infected with Toxoplasma gondii. Methods: Real-time PCR was used to measure the level of miRNA712_3p in plasma of infected mice. Immune-competent and immune-suppressed mice were examined, three and five days post-infection. Results: Results revealed significant up-regulation of plasma miRNA712_3p in both immune-competent and immune-compromised groups in comparison to the control non-infected group. Additionally, an increase in the level of miRNA712_3p was noticed correspondently in the parasite density detected in liver impression smears. Conclusions: miRNA712_3p can be used as a novel biomarker for the detection of Toxoplasma gondii infection in both immune-competent and immune-compromised host.