Screening of antiproliferative activity mediated through apoptosis pathway in human non-small lung cancer A-549 cells by active compounds present in medicinal plants

Author: Nutan V. BADGUJAR ¹ ; Kinnari N. MISTRY ¹ ; Dharamshibhai N. RANK ² ; Chaitanya G. JOSHI ³
Author Information

1. Ashok and Rita Patel Inst. of Intgd. Stud. and Res. in Biotechnology and Allied Sciences (ARIBAS), Affiliated to Sardar Patel University
2. Department of Animal Breeding and Genetics, College of Veterinary Science and Animal Husbandry, Anand Agricultural University
3. Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University
Publication Type:Journal Article
Keywords: Apoptosis; DAPI staining; DNA fragmentation assay; MTT assay; Real time assay; ROS
From: Asian Pacific Journal of Tropical Medicine 2018;11(12):666-675
CountryChina
Language:Chinese
Abstract: Objective: To explore the antiproliferative activity and apoptosis in cells caused by active compounds present in plants using different techniques. Methods: We investigated the antiproliferative effects of methanolic extracts from different parts of seven plants on A-549 (lung cancer) cells and primary cell culture (chick embryo fibroblast cells, as normal cells) using MTT assay and the potent plant was fractioned further. All these fractions were screened again for anti-proliferative activity. DNA fragmentation and DAPI staining were used to study apoptosis. Quantitative real-time was used to investigate the expression of apoptotic-related genes. LC-MS and