Characterization of the salivary microbiome in healthy Thai children

Izumi MASHIMA; Citra F THEODOREA; Boonyanit THAWEBOON; Sroisiri THAWEBOON; Tippanart VICHAYANRAT; Frank A SCANNAPIECO; Futoshi NAKAZAWA

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Characterization of the salivary microbiome in healthy Thai children

Author: Izumi MASHIMA ¹ ; Citra F. THEODOREA ² ; Boonyanit THAWEBOON ³ ; Sroisiri THAWEBOON ³ ; Tippanart VICHAYANRAT ⁴ ; Frank A. SCANNAPIECO ⁵ ; Futoshi NAKAZAWA ⁶
Author Information

1. Department of Microbiology, School of Pharmacy, Aichi Gakuin University
2. Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia
3. Department of Oral Microbiology, Faculty of Dentistry, Mahidol University
4. Department of Community Dentistry, Faculty of Dentistry, Mihidol University
5. Department of Oral Biology, School of Dental Medicine, University at Buffalo, State University of New York
6. Department of Microbiology, School of Dentistry, Health Sciences University of Hokkaido
Publication Type:Journal Article
Keywords: Children; Microbiome; Region; Saliva; Simplified oral hygiene index; Thai
From: Asian Pacific Journal of Tropical Medicine 2019;12(4):163-169
CountryChina
Language:Chinese
Abstract: Objective: To investigate the composition of the salivary microbiome of 50 healthy Thai children. Methods: A total 76 provinces in Thailand are grouped into 5 geographical clusters based on unique economics, foods and lifestyles. Geographical locations and the results of an oral assessment were also considered. Genomic DNA was extracted from stimulated sdiva samples. Subsequently, amplicon libraries were prepared by 16S Metagenomic Sequencing Library Preparation. The amplicons were sequenced using an Illumina Miseq platform followed by bioinformatics and statistical analyses. Results: The correlation between oral hygiene status and caries history varied from r