Tioxolone niosomes exert antileishmanial effects on Leishmania tropica by promoting promastigote apoptosis and immunomodulation

Maryam Hakimi Parizi; Iraj Sharifi; Ali Keyhani; Mahshid Mostafavi; Ahmad Khosravi; Daryoush Ghaffari; Saeedeh Farajzadeh; Abbas Pardakhty; Mohammad Parizi; Hamid Sharifi; Mehdi Bamorovat

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Tioxolone niosomes exert antileishmanial effects on Leishmania tropica by promoting promastigote apoptosis and immunomodulation

Author: Maryam HAKIMI PARIZI ¹ ; Iraj SHARIFI ¹ ; Ali KEYHANI ¹ ; Mahshid MOSTAFAVI ¹ ; Ahmad KHOSRAVI ¹ ; Daryoush GHAFFARI ¹ ; Saeedeh FARAJZADEH ² ; Abbas PARDAKHTY ³ ; Mohammad PARIZI ⁴ ; Hamid SHARIFI ⁵ ; Mehdi BAMOROVAT ⁶
Author Information

1. Leishmaniasis Research Center, Kerman University of Medical Sciences
2. Department of Pediatric Dermatology, Kerman University of Medical Sciences
3. Pharmaceutics Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences
4. Department of Pediatrics, Kerman University of Medical Sciences
5. HIV/STI Surveillance Research Center, WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences
6. Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences
Publication Type:Journal Article
Keywords: Apoptosis; Leishmania tropica; Niosome; Tioxolone
From: Asian Pacific Journal of Tropical Medicine 2019;12(8):365-374
CountryChina
Language:Chinese
Abstract: Objective: To explore the antileishmanial effect of tioxolone and its niosomal form against Leishmania tropica. Methods: Tioxolone niosomes were prepared by the hydration method and were evaluated for morphology, size, release study, and encapsulation efficiency. The cytotoxicity of tioxolone and its niosomal form was measured by MTT assay, leishmanicidal activity against promastigote and amastigote by MTT assay, apoptosis by flow cytometry, IL-12, IL-10 and metacaspase gene expression levels by q-PCR. Results: Span/Tween 40 and Span/Tween 60 niosomes had good physical stability as depicted in their size distribution curves and high encapsulation efficiency (>99%). The release profile of the entrapped compounds showed Fickian's model of tioxolone delivery based on diffusion through lipid bilayers. With the IC