Inhibitory effects of methanolic Olea europaea and acetonic Acacia laeta on growth of Babesia and Theileria

Author: Amany Magdy BESHBISHY ¹ ; Gaber EL-SABER BATIHA ¹ ; Naoaki YOKOYAMA ¹ ; Ikuo IGARASHI ¹ ; Gaber EL-SABER BATIHA ² ; Oluyomi Stephen ADEYEMI ³
Author Information

1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
2. Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University
3. Medicinal Biochemistry, Infectious Diseases, Nanomedicine and Toxicology Laboratory, Department of Biochemistry, Landmark University
Publication Type:Journal Article
Keywords: Acacia laeta; Babesia; In vitro; In vivo; Inhibition; Olea europaea; Theileria
From: Asian Pacific Journal of Tropical Medicine 2019;12(9):425-434
CountryChina
Language:Chinese
Abstract: Objective: To evaluate the antipiroplasmic activities of methanolic extract of Olea europaea (MOE) and acetonic extract of Acacia laeta (AAL) against Babesia and Theileria parasites in vitro and evaluate the chemotherapeutic effects of these extracts against Babesia (B.) microti in vivo. Methods: Fluorescence assay using SYBR Green 1 nucleic acid stain was used to detect inhibitory effects of the two extracts as well as the combination effects of the two extracts with diminazene aceturate and atovaquone on four Babesia species and Theileria equi in vitro while for in vivo experiments, 8-weekold female BALB/c mice were injected intraperitoneally with 1 × 10