Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran

Nabi JOMEHZADEH; Khadijeh AHMADI; Maryam AFZALI; Khadijeh AHMADI; Shokrollah SALMANZADEH; Fateme Jahangiri MEHR

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Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran

Author: Nabi JOMEHZADEH ¹ ; Khadijeh AHMADI ¹ ; Maryam AFZALI ² ; Khadijeh AHMADI ² ; Shokrollah SALMANZADEH ² ; Fateme Jahangiri MEHR ³
Author Information

1. Abadan Faculty of Medical Sciences
2. Infectious and Tropical Diseases Research Center, Health Research Institue, Ahvaz Jundishapur University of Medical Sciences
3. Biostatistics and Epidemiology Department, Ahvaz Jundishapur University of Medical Sciences
Publication Type:Journal Article
Keywords: Integrons; Multi-drug resistance; PCR; Shigella spp.
From: Asian Pacific Journal of Tropical Medicine 2021;14(2):78-82
CountryChina
Language:Chinese
Abstract: Objective: To investigate the antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran. Methods: In this study, 1 530 stool samples were collected from children under 15 years with diarrhea referred to teaching hospitals in Ahvaz and Abadan, southwest Iran. Shigella spp. were identified by standard biochemical tests and PCR. The antibiotic resistance pattern of all Shigella isolates was determined by the disk diffusion method and minimum inhibitory concentration (MIC) by E-test. Results: Of 1 530 stool samples, 91 (5.9%, 91/1 530) were positive for Shigella spp. the most common Shigella isolates were Shigella flexneri 47 (51.6%, 47/1 530). Antibiotic susceptibility tests showed that the highest antibiotic resistance was related to trimethoprim-sulfamethoxazole (87.9%, 80/91) and ampicillin (86.8%, 79/91). Multiplex PCR results revealed that 56% and 86.9% of Shigella isolates carried integron class I and integron class II genes, respectively. None of the isolates included the integron class III gene. Conclusions: The high prevalence of multi-drug resistance in Shigella isolates in our area increases the concerns about dissemination of the antibiotic-resistant isolates in this bacterium.