Detection of dengue virus serotype 3 in Cajamarca, Peru: Molecular diagnosis and clinical characteristics

Miguel Aguilar-luis; Wilmer Silva-caso; Yordi Tarazona-castro; Ronald Aquino-ortega; Angela Cornejo-tapia; Juana Valle-mendoza; Miguel Aguilar-luis; Wilmer Silva-caso; Yordi Tarazona-castro; Ronald Aquino-ortega; Juana Valle-mendoza; Hugo Carrillo-ng; Eduardo Verne; Sungmin Kym; Luis Del Valle; Jorge Bazn-mayra; Victor Zavaleta-gavidia; Daniel Cornejo-pacherres

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Detection of dengue virus serotype 3 in Cajamarca, Peru: Molecular diagnosis and clinical characteristics

Author: Miguel AGUILAR-LUIS ¹ ; Wilmer SILVA-CASO ¹ ; Yordi TARAZONA-CASTRO ¹ ; Ronald AQUINO-ORTEGA ¹ ; Angela CORNEJO-TAPIA ¹ ; Juana VALLE-MENDOZA ¹ ; Miguel AGUILAR-LUIS ² ; Wilmer SILVA-CASO ² ; Yordi TARAZONA-CASTRO ² ; Ronald AQUINO-ORTEGA ² ; Juana VALLE-MENDOZA ² ; Hugo CARRILLO-NG ³ ; Eduardo VERNE ³ ; Sungmin KYM ⁴ ; Luis DEL VALLE ⁵ ; Jorge BAZN-MAYRA ⁶ ; Victor ZAVALETA-GAVIDIA ⁶ ; Daniel CORNEJO-PACHERRES ⁶
Author Information

1. School of Medicine, Research and Innovation Center of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas
2. Laboratorio de Biologia Molecular, Instituto de Investigaci n Nutricional
3. Facultad de Medicina Alberto Hurtado, Universidad Peruana Cayetano Heredia
4. Division of Infectious Diseases, Department of Internal Medicine, Chungnam National University, School of Medicine
5. Barcelona Research Center for Multiscale Science and Engineering, Departament d'Enginyeria Qu mica, EEBE, Universitat Polit cnica de Catalunya (UPC)
6. Laboratorio Regional de Cajamarca, Direcci n Regional de Salud de Cajamarca (DIRESA)
Publication Type:Journal Article
Keywords: Arbovirus; Dengue; DENV; Diagnosis; Peru
From: Asian Pacific Journal of Tropical Medicine 2021;14(10):456-462
CountryChina
Language:Chinese
Abstract: Objective: To describe and molecularly characterize an outbreak of dengue virus (DENV) infection in Cajamarca, an Andean region in Peru. Methods: A total of 359 serum samples from patients with acute febrile illness were assessed for the presence of DENV via RT-PCR, ELISA NS1, IgM and IgG in Cajamarca, Peru from January 2017 to June 2017. The evaluation of the different diagnostic tests and their applicability was performed. Results: Dengue virus was detected in 24.7% of samples by RTPCR. Meanwhile, serological analysis detected 30.3% positive cases via ELISA NS1 antigen, 16.7% via ELISA IgG and 9.7% via ELISA IgM. Most of the cases corresponded to DENV-3 (77.5%). The use of RT-PCR performed better in primary infections (P<0.01), while detection of ELISA IgM performed better in secondary infections (P<0.01). The combination of NS1 and IgM performed better than the other assays in detecting primary (92.5%) and secondary infections (96.6%). The most frequent symptoms associated with fever were headaches, myalgias, and arthralgias across all groups. Conclusions: We report an important outbreak of dengue infection caused by DENV-3 in Cajamarca, Peru. Our findings encourage the use of NS1 antigen and IgM co-detection. These findings demonstrate an increasing expansion of DENV-3 in Peru and highlight the importance of molecular diagnosis and serotype characterization among the clinically defined dengue cases to strengthen the Peruvian epidemiological surveillance.