Prevalence of non-tuberculosis mycobacteria among samples deposited from the National Tuberculous Reference Laboratory of Iran (2011-2018)

Saman Ayoubi; Parissa Farnia; Jafar Aghajani; Jalaledin Ghanavi; Ali Velayati; Poopak Farnia

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Prevalence of non-tuberculosis mycobacteria among samples deposited from the National Tuberculous Reference Laboratory of Iran (2011-2018)

Author: Saman AYOUBI ¹ ; Parissa FARNIA ¹ ; Jafar AGHAJANI ¹ ; Jalaledin GHANAVI ¹ ; Ali VELAYATI ¹ ; Poopak FARNIA ²
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1. Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences
2. Department of Biotechnology, School of Advanced Technology in Medicine, Shahid Beheshti University of Medical Sciences
Publication Type:Journal Article
Keywords: Diagnosis; Iran; Non-tuberculosis Mycobacterium; Prevalence
From: Asian Pacific Journal of Tropical Medicine 2021;14(10):451-455
CountryChina
Language:Chinese
Abstract: Objective: To investigate the prevalence of non-tuberculosis mycobacteria (NTM) among the samples deposited from the National Tuberculosis Reference Laboratory of Iran between 2011 and 2018. Methods: The study evaluated the prevalence of NTM among specimens from patients with pulmonary tuberculosis symptoms (n=15 771) deposited at the National Tuberculosis Reference Laboratory of Iran from 2011 to 2018. Detection of Mycobacterium (M.) tuberculosis was based on presence of a 190-bp amplicon from IS6110 insertion sequence using Tb1 and Tb2 primers, and amplicon-negative specimens were tested for NTM and M. tuberculosis (refractory to IS6110 amplification) using restriction fragment length polymorphism PCR of hsp65 amplicon fragment. Results: A total of 7 307 (46.33%) M. tuberculosis and 658 (4.17%) NTM specimens were found, the latter mainly comprising M. abscessus (10.18%), M. avium (2.28%), M. chelonae (8.97%), M. intracellulare (10.49%), M. kansasii (4.71%), and M. simiae (56.08%). Conclusions: As treatment for NTM differs from that for M. tuberculosis, accurate detection of Mycobacterium sp. is of public health significance.