Result of Transplantation of Chondrocytes and Periosteum-Derived Cells for Long-Bone Growth Plate Injuries of the Rabbits.
- Author:
Hui Taek KIM
1
;
Dong Joon KANG
;
Wang Jun KIM
;
Hee Kyung CHANG
;
Chong Il YOO
Author Information
1. Department of Orthopaedic Surgery, College of Medicine, Pusan National University, Busan, Korea. kimht@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Growth plate injury;
Chondrocyte;
Periosteum-derived cell
- MeSH:
Agar;
Cell Transplantation;
Chondrocytes*;
Congenital Abnormalities;
Femur;
Gelatin Sponge, Absorbable;
Genu Varum;
Growth Plate*;
Rabbits*;
Tibia;
Transplants
- From:Journal of Korean Orthopaedic Research Society
2005;8(2):111-120
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To study the effectiveness of chondrocyte and periosteum-derived cell transplantation in preventing bar formation in growth plate injuries. MATERIALS AND METHODS: Thirty immature rabbits were grouped according to site of growth plate damaged (distal femur or proximal tibia on the medial side) and type of cell and material inserted (chondrocytes plus agar, periosteum-derived cells plus agar, periosteum-derived cells plus Gelfoam, agar alone, or Gelfoam alone). Radiograms were taken for 16 weeks to measure the changes in varus angle of the distal femur and proximal tibia. Transformation of inserted cells was observed histologically. RESULTS: Rabbits inserted with agar alone showed more severe genu varum compared to those inserted with chondrocytes plus agar. Rabbits inserted with periosteum-derived cells plus agar or Gelfoam showed similar deformities compared to those inserted with agar or Gelfoam alone. Some inserted periosteum-derived cells began transformation into chondrocytes. All rabbits showed gradual bar formation and ultimate fusion of the growth plate. CONCLUSION: Cell-based therapy of growth plate injury still has obstacles to overcome. Further study will be required on how to maintain the inserted chondrocytes, or chondrocytes differentiated from the inserted periosteum-derived cells, and to prevent them from changing into osteoid formation cells.
