Antimicrobial susceptibility and serotypes of Neisseria meningitidis and Streptococcus pneumoniae in Sri Lanka: Experience from the National Reference Laboratory

Lilani KARUNANAYAKE; Lilani GUNAWARDANA; Vayishnavi ARIRAM; Veeraraghavan BALAJI; Rosemol VARGHESE; Malka DASSANAYAKE; Vaithehi FRANCIS; Lakmini YAPA; Chamika HERATH; Nambage CHANDRASIRI; Priyanka WIMALAGUNAWARDHANA

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Antimicrobial susceptibility and serotypes of Neisseria meningitidis and Streptococcus pneumoniae in Sri Lanka: Experience from the National Reference Laboratory

Author: Lilani KARUNANAYAKE ¹ ; Lilani GUNAWARDANA ¹ ; Vayishnavi ARIRAM ¹ ; Veeraraghavan BALAJI ² ; Rosemol VARGHESE ² ; Malka DASSANAYAKE ³ ; Vaithehi FRANCIS ⁴ ; Lakmini YAPA ⁵ ; Chamika HERATH ⁶ ; Nambage CHANDRASIRI ⁷ ; Priyanka WIMALAGUNAWARDHANA ⁶
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1. Department of Bacteriology, Medical Research Institute
2. Department of Microbiology, Christian Medical College
3. Lady Ridgeway Children's Hospital
4. Department of Pathophysiology, Faculty of Healthcare Sciences, Eastern University
5. Teaching Hospital
6. District General Hospital
7. Colombo South Teaching Hospital
Collective Name:North Colombo Teaching Hospital;District General Hospital
Publication Type:Journal Article
Keywords: Antimicrobial resistance; Invasive bacterial diseases; Neisseria meningitidis; Serotypes; Sri Lanka; Streptococcus pneumoniae
From: Asian Pacific Journal of Tropical Medicine 2022;15(3):114-120
CountryChina
Language:Chinese
Abstract: Objective: To determine the antimicrobial susceptibility and serotypes of Neisseria (N.) meningitidis and Streptococcus (S.) pneumoniae in Sri Lankan patients. Methods: We retrospectively analyzed 11 blood culture specimens from suspected patients with invasive meningococcal disease and 26 S. pneumoniae clinical isolates. We tested 6 antimicrobials against N. meningitidis and 12 antimicrobials against S. pneumoniae. Meningococcal serogroup was determined by realtime PCR and Quellung serotyping was used for pneumococcal analysis. Results: N. meningitidis serogroup B was the most common in this study. Intermediate-susceptibility to penicillin was seen in 75.0% (6/8) of strains. Susceptibility to ciprofloxacin, levofloxacin and cotrimoxazole was 62.5% (5/8), 62.5% (5/8) and 87.5% (7/8), respectively. Excellent susceptibility was seen in cefotaxime and meropenem. In S. pneumoniae, the most common serotype was 19F in both invasive and non-invasive pneumococcal diseases. The majority of strains showed multidrug resistance. Penicillin non-susceptibility in non-meningeal strains were 13.6% and all meningeal strains were penicillin resistant. Erythromycin was highly resistant in both groups. Amoxicillin showed excellent susceptibility in non-invasive pneumococcal diseases strains. Linezolid, levofloxacin and vancomycin showed 100.0% susceptibility in all pneumococcal isolates. Conclusions: Implementation of vaccines should be considered, especially for children and high-risk populations. This may contribute to reducing pneumococcal and meningococcal invasive disease burden and help prevent emergence of antimicrobial resistant strains.