Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells
10.1016/S2221-1691(15)30372-5
- Author:
Maram SHALABI
1
;
Mahmoud G. ELSEBAEI
1
;
Kh. KHILO
2
;
Mahmoud M. ZAKARIA
3
;
Walied ABDO
4
;
Walaa AWADIN
5
Author Information
1. Department of Biochemistry, Faculty of Veterinary Medicine, Mansoura University
2. Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University
3. Urology and Nephrology Center, Mansoura University
4. Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University
5. Department of Pathology, Faculty of Veterinary Medicine, Mansoura University
- Publication Type:Journal Article
- Keywords:
Aloe vera;
Apoptosis;
Calligonum comosum;
Hepatocellular carcinoma;
HepG2
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(5):375-381
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the in vitro anticancer effect of Aloe vera (A. vera) and Calligonum comosum (C. comosum) extracts against hepatocellular carcinoma (HepG2) cells. METHODS: HepG2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic (Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG2 cells were observed using light microscopy. RESULTS: The IC
