Study on the stable of gene sequence of seed lot system G1P8 KH0118 during production rotavirus vaccine
- Author:
Luan Thi Le
;
Hien Dang Nguyen
;
Huong Thi Mai Nguyen
- Publication Type:Journal Article
- Keywords:
rotavirus vaccine;
G1P8;
gene sequence
- MeSH:
rotavirus vaccine;
G1P8;
gene sequence
- From:Journal of Preventive Medicine
2008;18(6):67-73
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Background: Rotavirus strain (KH0118) is used as the primary material to produce original rotavirus vaccine strains with the symbol of G1P8 MS. According to the World Health Organization\u2019s standard, the strain is needed to evaluate the stability of gene throughout analysis of gene and amino acid sequence during vaccine production. Objective: To determine the sequence of genes 4 (VP4), 6 (VP6), 9 (VP7) and 10 (NSP4) with base pair correlative 855:866:1345:745 of seed lot system and vaccine of G1P8 strain and to evaluate the stability of seed lot system during vaccine production. Subject and methods: ARN was divided from the original strain of rotavirus vaccine G1P8 MS, rotavirus vaccine productive strain (G1P8 WS) and rotavirus vaccine (G1P8 VX). Then using primer pairs to determine gene sequence VP4, VP6, VP7, NSP4 and comparing gene and amino acid sequence of the seed lot system. Results and Conclusion: The study demonstrated that, there was no difference for the nucleotide and amino acid sequence from the original strain during production of rotavirus vaccine G1P8 KH0118.
