Anti-diabetic potential of Urena lobata leaf extract through inhibition of dipeptidyl peptidase IV activity
10.1016/j.apjtb.2015.05.014
- Author:
Yudi PURNOMO
1
;
Yudi PURNOMO
2
;
Djoko Wahono SOEATMADJI
3
;
Sutiman Bambang SUMITRO
4
;
Mochamad Aris WIDODO
5
Author Information
1. School of Medicine, Brawijaya University
2. Pharmacology Department, School of Medicine, Malang Islamic University
3. Internal Department, School of Medicine, Brawijaya University
4. Biology Department, Faculty of Science, Brawijaya University
5. Pharmacology Department, School of Medicine, Brawijaya University
- Publication Type:Journal Article
- Keywords:
Anti-diabetic;
Dipeptidyl peptidase IV;
In silico;
In vitro;
Urena lobata
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(8):645-649
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the anti-diabetic potential of leaf extract from Urena lobata (U. lobata) through dipeptidyl peptidase IV (DPP-IV) inhibitory activity. Methods: U. lobata leaf was extracted in hot water and ethanol. The activity of DPP-IV inhibitor was tested by in vitro study using gly-pro-p-nitroanilide as substrat of DPP-IV and vildagliptin, as standard reference. A product of the reactions between gly-pro-pnitroanilide and DPP-IV, was observed by microplate readers with λ = 405 nm. All data were expressed as mean ± SD and the IC50 value was determined by non linear regression curve fit. Active substances in leaf extract of U. lobata was analyzed by liquid chromatography-mass spectrometry. DPP-IV inhibitory activity of active compounds was evaluated in silico using docking server. Results: The ethanolic extract of U. lobata showed stronger DPP-IV inhibitor activity than water extract with the IC50 values of 1 654.64 and 6 489.88 mg/mL, respectively. Vildagliptin, based on standard reference for DPP-IV inhibitor activity, has IC50 value of 57.44 μg/mL. Based on in silico analysis, mangiferin, stigmasterol and β-sitosterol in U. lobata extract have a strong inhibitory activity on DPP-IV. Conclusions: The results showed that DPP-IV inhibitory activity of U. lobata is related to its active compounds such as mangiferin, stigmasterol and β-sitosterol.
