Evaluation of zoonotic potency of Escherichia coli O157: H7 through arbitrarily primed PCR methods
10.1016/j.apjtb.2015.07.023
- Author:
I. Wayan SUARDANA
1
;
Dyah Ayu WIDIASIH
2
;
I. Gusti Ngurah Kade MAHARDIKA
3
;
Komang Januartha Putra PINATIH
4
;
Budi Setiadi DARYONO
5
Author Information
1. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University
2. Department of Veterinary Public Health, Faculty of Veterinary Medicine, Gadjah Mada University
3. Department of Virology, Faculty of Veterinary Medicine, Udayana University
4. Department of Clinical Microbiology, Faculty of Medicine, Udayana University
5. Department of Genetic, Faculty of Biology, Gadjah Mada University
- Publication Type:Journal Article
- Keywords:
Animals;
AP-PCR;
E. coli O157:H7;
Human;
Zoonoses
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(11):915-920
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the zoonotic potency of Escherichia coli O157:H7 through arbitrarily primed-PCR (AP-PCR) methods as one of the DNA fingerprinting methods. Methods: A total of 14 isolates consisted of 11 isolates originated from human feces with renal failure symptoms, 2 isolates originated from cattle feces, and 1 control isolate were used in this study. DNA of each isolate was extracted, and their profiles were studied by using AP-PCR method with M13 F and M13 R arbitrary primers. Results: The results founded that all of 14 isolates had similarity range from 54.6% to 88.5%. Isolates KL-106(3) and KL-55(6) originated from humans showed the degree of similarity with isolates SM-25(1) and SM-7(1) originated from cattle as high as 85% and 77%, respectively. Conclusions: The high degree of similarity between isolates originated from cattle and human indicated the high potency of zoonoses. The results also concluded AP-PCR method as a briefly fingerprinting method in order to trace the epidemiological of E. coli O157:H7.
