A novel HPTLC method for quantitative estimation of biomarkers in polyherbal formulation

Author: Zeeshan Ahmed SHEIKH ¹ ; Aqib ZAHOOR ¹ ; Saleha Suleman KHAN ¹ ; Khan USMANGHANI ¹ ; Sadia SHAKEEL ² ; Somia GUL ³ ; Khan USMANGHANI ³ ; Faisal Haider ZAIDI ⁴
Author Information

1. Herbion Pakistan (Pvt.) Ltd.
2. Dow College of Pharmacy, Dow University of Health Sciences
3. Faculty of Pharmacy, Jinnah University for Women Karachi
4. Department of Basic Medical Sciences, College of Medicine, King Saud bin Abdulaziz University of Health Sciences
Publication Type:Journal Article
Keywords: Berberine; Entoban syrup; Gallic acid; HPTLC; Quantitative estimation
From:Asian Pacific Journal of Tropical Biomedicine 2015;5(11):955-959
CountryChina
Language:Chinese
Abstract: Objective: To explore the quantitative estimation of biomarkers gallic acid and berberine in polyherbal formulation Entoban syrup. Methods: High performance thin layer chromatography was performed to evaluate the presence of gallic acid and berberine employing toluene: ethyl acetate: formic acid: methanol 12:9:4:0.5 (v/v/v/v) and ethanol: water: formic acid 90:9:1 (v/v/v), as a mobile phase respectively. Results: The R