The leaf extract of Mallotus japonicus and its major active constituent, rutin, suppressed on melanin production in murine B16F1 melanoma
10.1016/j.apjtb.2015.05.017
- Author:
Junsei TAIRA
1
;
Eito TSUCHIDA
1
;
Masatsugu UEHARA
1
;
Natsuko OHHAMA
1
;
Wakana OHMINE
1
;
Takayuki OGI
2
Author Information
1. Department of Bioresource Technology, Okinawa National College of Technology
2. Okinawa Industrial Technology Center
- Publication Type:Journal Article
- Keywords:
Mallotus japonicus;
Melanin;
Melanogenesis;
Polyphenol;
Rutin;
Tyrosinase
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(10):819-823
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To find anti-melanogenesis materials used in whitening cosmetics. Methods: The ethanolic leaf extract of Mallotus japonicus (M. japonicus) having an anti-melanogenesis activity was separated by a sephadex LH-20 chromatography. Each fraction was measured for its tyrosinase inhibitory activity together with its polyphenol content using the Folin-Ciocalteu method. The anti-melanogenesis activity of the active fractions was determined by the melanin content in the murine B16F1 melanoma. The active fractions were put together due to similar constituents, and then separated by high performance liquid chromatography using a C-18 ODS column. The major anti-melanogenesis compound was identified using
