Immunomodulatory effects of selected Malaysian plants on the CD18/11a expression and phagocytosis activities of leukocytes
10.1016/S2221-1691(15)30170-2
- Author:
Nurul Hikmah HARUN
1
;
Abdi Wira SEPTAMA
2
;
Ibrahim JANTAN
3
Author Information
1. School of Biomedicine and Diagnostics, Faculty of Medicine and Health Sciences, Universiti Sultan Zainal Abidin
2. Faculty of Pharmacy, University of Sumatera Utara
3. Faculty of Pharmacy, Universiti Kebangsaan Malaysia
- Publication Type:Journal Article
- Keywords:
CD18/11a expression;
Flow cytometry analysis;
Immunomodulatory effects;
Phagocytosis activity;
Selected Malaysian medicinal plants
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of 20 methanolic extracts from Malaysian selected plants on CD18/11a expression and phagocytosis activity of leukocytes using flow cytometry analysis. Methods: The effects of methanolic extracts on CD18/11a expression and phagocytosis of leukocytes were measured by labelling the cells with CD18-fluorescein isothiocyanate and ingestion labelled with Escherichia coli-fluorescein isothiocyanate and then analyzed using flow cytometer. Results: About 12 out of 20 methanolic extracts of selected Malaysian medicinal plants significantly (P≤0.05) inhibited the CD18/11a expression of leukocytes at both concentrations of 6.25 μg/mL and 100 μg/mL in dose dependent manner. The most active inhibitory was shown in Citrus aurantifolia (Christm.) Swingle and Alpinia galangal (L.) Willd. at dosage 100 μg/mL. Moreover, the Orthosiphon aristatus (Blume) Miq (O. aristatus). showed the highest stimulatory activity at the concentration of 100 μg/mL. Other than that, four plant extracts significantly (P≤0.05) rose the phagocytosis activities of leukocytes in dose dependent manner. However, Annona muricata L. and O. aristatus showed the highest stimulated activities at the 100 μg/mL concentration. Conclusions: The results suggest that methanolic extracts of Citrus aurantifolia, Alpinia galangal, O. aristatus and Annona muricata are able to modulate innate immune system and can potentially be recognized as therapeutic agents for modulating immune system.
