Quantification of total phenol, flavonoid content and pharmacognostical evaluation including HPTLC fingerprinting for the standardization of Piper nigrum Linn fruits
10.1016/S2221-1691(15)30152-0
- Author:
Aftab AHMAD
1
;
Aftab AHMAD
2
;
Anil BHANDARI
2
;
Asif HUSAIN
3
;
Mohd MUJEEB
4
;
Shah Alam KHAN
5
;
Hani Abdullah Anber ALHADRAMI
6
Author Information
1. Health Information Technology Department, Jeddah Community College, King Abdulaziz University
2. Faculty of Pharmaceutical Sciences, Jodhpur National University
3. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hamdard University
4. Department of Pharmacognosy, Faculty of Pharmacy, Jamia Hamdard
5. Department of Pharmacy, Oman Medical College
6. Department of Medical Laboratory Technology, Faculty of Applied Medical Science, King Abdulaziz University
- Publication Type:Journal Article
- Keywords:
Black pepper;
HPTLC fingerprint;
Piper nigrum L. fruits;
Piperaceae
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(2):101-107
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To carry out the physicochemical and phytochemical standardization with high performance thin layer chromatography fingerprinting of Piper nigrum L. (P. nigrum) fruits in order to ascertain the standard pharmacognostical parameters of this king of spices. Methods: Many standardization parameters like extractive values, total ash value, water soluble ash value and acid insoluble ash, moisture content, loss on drying and pH values of P. nigrum L. fruits were analyzed. The method of Harborne was adopted for the preliminary phytochemicals screening. Analysis of total phenolic and flavonoid contents, pesticides residues, aflatoxin and heavy metals were also performed. CAMAG-high performance thin layer chromatography system was used for fingerprinting of methanolic extract of P. nigrum L. fruits. Results: The results of phytochemicals testing indicated the presence of carbohydrates, phenolic compounds, flavonoids, alkaloids, proteins, saponins, lipids, sterols and tannins in various solvent extracts. Total phenolic and flavonoid contents in methanolic extract were found to be 1.728 1 mg/g and 1.087 μg/g, respectively. Heavy metals concentrations were found to be within standard limits. Aflatoxins and pesticides residues were absent. Conclusions: The outcome of this study might prove beneficial in herbal industries for identification, purification and standardization of P. nigrum L. fruits.
