Morphological and molecular characterization of fungus isolated from tropical bed bugs in Northern Peninsular Malaysia, Cimex hemipterus (Hemiptera: Cimicidae)
10.1016/j.apjtb.2015.04.012
- Author:
Abdul Hafiz AB MAJID
1
;
Zulaikha ZAHRAN
1
;
Abd Hafis ABD RAHIM
1
;
Nor Azliza ISMAIL
2
;
Wardah ABDUL RAHMAN
2
;
Kartiekasari Syahidda MOHAMMAD ZUBAIRI
2
;
Hamady DIENG
3
;
Tomomitsu SATHO
4
Author Information
1. Household and Structural Urban Entomology Laboratory, School of Biological Sciences, Universiti Sains Malaysia
2. Plant Pathology Laboratory, School of Biological Sciences, Universiti Sains Malaysia
3. Institute of Biodiversity and Environmental Conservation, Universiti Malaysia Sarawak
4. Department of Microbiology, Faculty of Pharmaceutical Sciences, Fukuoka University
- Publication Type:Journal Article
- Keywords:
Aspergillus;
Cimex hemipterus;
Internal transcribed spacer;
Morphological characteristics;
Trichoderma;
β-tubulin
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(9):707-713
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate some morphological and molecular characteristics of fungal parasites isolated from wild tropical bed bug, Cimex hemipterus. Methods: A series of culture methods were used to obtain fungal isolates from field-collected bed bugs. Characteristics of the isolates such as colony appearance, mycelial texture and pigmentation were studied to explore their morphology. Isolates were also subjected to a PCR-based genotyping test. Results: There were noticeable differences in morphological characteristics among the four isolates. Conidial areas of one isolate were dark green, whereas those of the remaining colonies were olive-green, black or dark brown. Conidia of the dark green isolate were globose, while those of olive-green, black and dark brown isolates were globose to subglobose, globose to spherical and globose to subglobose/finely roughened, respectively. These morphological specificities and the molecular analyses showed that the fungal internal transcribed spacer ribosomal region and β-tubulin gene sequences of the isolates shared clade with Trichoderma and Aspergillus sequences. Conclusions: Overall, the new discovery of common pathogens in agricultural field developed in live bed bugs storage tank may initiate the use of biological agents in later years.
