Tinospora crispa extract inhibits MMP-13 and migration of head and neck squamous cell carcinoma cell lines
10.1016/j.apjtb.2015.07.001
- Author:
Hataipan PHIENWEJ
1
;
Ih-si SWASDICHIRA
1
;
Prasit PAVASANT
1
;
Piyamas SUMREJKANCHANAKIJ
1
;
Surattana AMNUOYPOL
2
;
Prasit PAVASANT
3
;
Piyamas SUMREJKANCHANAKIJ
3
Author Information
1. Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University
2. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University
3. Department of Anatomy, Faculty of Dentistry, Chulalongkorn University
- Publication Type:Journal Article
- Keywords:
Cell line;
Cell migration;
Matrix metalloproteinase-13;
Phytochemical;
Squamous cell carcinoma;
Tinospora crispa
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(9):738-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Tinospora crispa (T. crispa) extract on matrix metalloproteinase-13 (MMP-13) expression and cell migration. Methods: The cytotoxicity of T. crispa extract was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay on head and neck squamous cell carcinoma (HNSCC) cell lines. The effect on expression of MMP-13 was analysed by RT-PCR and ELISA. The migration was assessed by wound healing assay. Results: MMP-13 mRNA was highly expressed in the metastatic human HNSCC cell lines, HN22 and HSC-3. T. crispa extract at a concentration of 100.0 μg/mL caused about 50% reduction of cell survival. T. crispa extract at a non-toxic concentration of 12.5, 25.0 and 50.0 μg/mL significantly suppressed MMP-13 mRNA expression and secreted MMP-13 in both HN22 and HSC-3. The expression of tissue inhibitors of metalloproteinase-2 (TIMP-2) by HSC-3 cells was attenuated by 25.0 and 50.0 μg/mL of T. crispa extract. Addition of the extract to cells in a wound healing assay showed inhibition of cell migration by HN22 cells. Conclusions: These data suggest that T. crispa could be considered as a potential therapeutic drug to prevent metastasis of HNSCC.
