Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharideinduced acute liver injury in mice
10.1016/j.apjtb.2015.07.007
- Author:
Hai Nguyen THANH
1
;
Tuan Anh LE
1
;
Huong Duong Thi LY
1
;
Tung Nguyen HUU
1
;
Loi Vu DUC
1
;
Thu Dang KIM
1
;
Tung Bui THANH
1
;
Hue Pham Thi MINH
2
Author Information
1. School of Medicine and Pharmacy, Vietnam National University
2. Hanoi University of Pharmacy
- Publication Type:Journal Article
- Keywords:
Cytokine;
Lipopolysaccharide;
Liver injury;
Nuclear factor-κB;
Scutellaria baicalensis
- From:Asian Pacific Journal of Tropical Biomedicine
2015;5(9):761-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis (S. baicalensis) against lipopolysaccharide (LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS (5 mg/kg of body weight, intraperitoneal injection). Both protein and mRNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. Cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-kB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS significantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver mice. Conclusions: Our study showed that S. baicalensis is potentially protective against LPSinduced liver injury in mice.
