The inhibition of Typhonium flagelliforme Lodd. Blume leaf extract on COX-2 expression of WiDr colon cancer cells
10.1016/j.apjtb.2015.12.012
- Author:
Agustina SETIAWATI
1
;
Handika IMMANUEL
1
;
Mery Tri UTAMI
1
Author Information
1. Department of Drug Design and Discovery, Faculty of Pharmacy, Sanata Dharma University
- Publication Type:Journal Article
- Keywords:
Colon cancer;
Cyclooxygenase 2;
Typhonium flagelliforme;
WiDr
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine the inhibition activity of Typhonium flagelliforme Lodd. Blume (T. flagelliforme) leaf extract on cyclooxygenase 2 (COX-2) expression of colon cancer cells. Methods: T. flagelliforme leaf extract was prepared to macerate in ethyl acetate. In vitro anticancer activity was assayed by MTT method on WiDr colon cancer cells. This study applied apoptosis induction assay to investigate the mechanism of cell death using double staining method. COX-2 expression was stained by immunocytochemistry. Results: T. flagelliforme showed anticancer activity and induced apoptosis on WiDr cells through inhibition of COX-2 expression with IC
