In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis
10.1016/j.apjtb.2015.12.001
- Author:
Jafar JALAEI
1
;
Mehdi FAZELI
1
;
Hamid RAJAIAN
1
;
Somayeh Layeghi GHALEHSOUKHTEH
1
;
Alireza DEHGHANI
2
;
Dominic WINTER
2
Author Information
1. Department of Pharmacology and Toxicology, School of Veterinary Medicine, Shiraz University
2. Institute of Biochemistry and Molecular Biology, University of Bonn
- Publication Type:Journal Article
- Keywords:
Antihistamine release;
Lactate dehydrogenase assay;
Peptide;
Vespa orientalis
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(3):259-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis. Methods: This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed. Results: No significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC
