Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action
10.1016/j.apjtb.2015.08.004
- Author:
Meriem EL KOLLI
1
;
Hocine LAOUER
1
;
Farida SAHLI
1
;
Salah AKKAL
2
;
Hayet EL KOLLI
3
Author Information
1. Laboratory of Natural Biological Resources, University of Sétif 1
2. VARENBIOMOL: Department of Chemistry, University of Constantine1
3. Laboratory of Multiphase Polymeric Materials, University of Sétif 1
- Publication Type:Journal Article
- Keywords:
Anti-oxidative activity;
Antimicrobial activity;
Apiaceae;
Daucus gracilis;
Essential oils;
Macro-dilution;
Micro-dilution;
Reducing power
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(1):8-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxidant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry, and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method. The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience. The minimal inhibitory concentrations were determined by agar macro-dilution and micro-dilution methods. Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques. Results: The EO yielded 0.68 (v/w). The chemical analysis presented two dominant constituents which were the elemicin (35.3%) and the geranyl acetate (26.8%). D. gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and 57.05 μg/mL and 114.1 μg/mL, respectively by liquid micro-dilution. A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded, which suggested that the cytoplasm membrane was one of the targets of the EO. The EO showed, also, important anti-oxidative effects with an IC
