Evaluation of entomopathogenic Bacillus sphaericus isolated from Lombok beach area against mosquito larvae
10.1016/j.apjtb.2015.10.013
- Author:
Bambang Fajar SURYADI
1
;
Bambang Fajar SURYADI
2
;
Bagyo YANUWIADI
2
;
Tri ARDYATI
2
;
Suharjono SUHARJONO
2
Author Information
1. Department of Biology, Faculty of Mathematics Natural Sciences, Mataram University
2. Department of Biology, Faculty of Mathematics Natural Sciences, Brawijaya University
- Publication Type:Journal Article
- Keywords:
Aedes aegypti;
Anopheles aconitus;
Bacillus sphaericus;
Culex quinquefasciatus;
Lombok Island;
Mosquito larvae
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(2):148-154
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate, characterize and evaluate toxicity of Bacillus sphaericus (B. sphaericus) from beach area of Lombok Island. Methods: Soil was collected from determined locations and suspended in sterile physiological saline water. After heat shock was applied, suspension was spread on NYSM agar medium. Colonies grown were then observed and isolated. Colony, cell morphology, and biochemical/physiological characteristics were tested and compared to B. sphaericus 2362 as standard. Initial toxicity testing was done against three species of mosquito larvae (Culex quinquefasciatus, Anopheles aconitus and Aedes aegypti) and isolates that showed more than 50% larvae killing will be assayed to obtain LC
