Sulforaphene and sulforaphane in commonly consumed cruciferous plants contributed to antiproliferation in HCT116 colon cancer cells
10.1016/j.apjtb.2015.11.003
- Author:
Piman POCASAP
1
;
Natthida WEERAPREEYAKUL
2
Author Information
1. Graduate School, Faculty of Pharmaceutical Sciences, Khon Kaen University
2. Faculty of Pharmaceutical Sciences, Khon Kaen University
- Publication Type:Journal Article
- Keywords:
Cancer;
Chemoprevention;
Cruciferous;
Isothiocyanates;
Sulforaphane;
Sulforaphene
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(2):119-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze two isothiocyanates (sulforaphene and sulforaphane) and their antiproliferative effect of 11 indigenous cruciferous vegetables. Methods: Phytoconstituents identification was conducted by high performance liquid chromatography and gas chromatography-mass spectrometer techniques. The antiproliferation was evaluated in colon cancer cell line HCT116 by MTT assay. Results: Isothiocyanate identification by high performance liquid chromatography showed that broccoli, cabbage, "Khi-Hood" (Raphanus sativus L. var. caudatus Alef) and Chinese radish contained isothiocyanates sulforaphane. Sulforaphene and sulforaphane in broccoli, cabbage and "Khi-Hood" were characterized by the gas chromatography-mass spectrometer analysis. Antiproliferation screening by MTT assay found that the potent plants which possessed IC
