Neurotropic effects of aspartame, stevia and sucralose on memory retention and on the histology of the hippocampus of the ICR mice (Mus musculus)
10.1016/j.apjtb.2015.11.001
- Author:
Lejan Miguel Alabastro VILLAREAL
1
;
Rachelle Anne Montes CRUZ
1
;
Michael Bagui PLES
1
;
Rodel Jonathan Santos VITOR
1
Author Information
1. Biology Department, College of Science, De La Salle University
- Publication Type:Journal Article
- Keywords:
Aspartame;
Hippocampus;
Learning;
Memory;
Mus musculus;
Stevia;
Sucralose
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(2):114-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the effects of the consumption of non-nutritive sweeteners on memory retention and on the histology of the hippocampus. Methods: In this study, 20 mice were used to determine if there is an effect of consuming the maximum allowable dose of the non-nutritive sweeteners on the memory retention and on the histology of the hippocampus. The mice were distributed into four groups and the treatments were given via oral gavage: Group 1 (water), Group 2 (aspartame: 1. 000 mg/kg), Group 3 (stevia: 1. 000 mg/kg) and Group 4 (sucralose: 16. 000 mg/kg). Treatments were administered to the different experimental groups for 32 days, after which memory retention was tested using the two-day water maze protocol. After the tests, the mice were sacrificed and the brain was analyzed histologically for neurotrophic effects. Results: Based on the results of the two-day water maze protocol, there were no differences between the non-nutritive sweeteners and the control group. However, stevia showed high cellular apoptosis followed by aspartame, sucralose and control group. Conclusions: There was no significant effect on the memory of the mice. It showed histologically however, that stevia had a significant neurotropic effect compared to the other sweeteners.
