Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production
10.1016/j.apjtb.2015.12.018
- Author:
Louise Helena GUIMARÃES DE OLIVEIRA
1
;
Patricia ALEXANDRIA PAIVA SILVA DE SOUSA
1
;
Fabrine FELIPE HILARIO
1
;
Gabriel JOVENTINO NASCIMENTO
1
;
Fabiola DA CRUZ NUNES
1
;
João Paulo Saraiva MORAIS
2
;
Everaldo PAULO DE MEDEIROS
2
;
Manoel FRANCISCO DE SOUSA
2
Author Information
1. Biotechnology Center, Federal University of Paraiba
2. Embrapa Algodão, Brazilian Agricultural Research Corporation
- Publication Type:Journal Article
- Keywords:
Cell culture;
Insecticide;
Mosquito;
Sisal
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(5):396-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture. Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18-6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured. Results: Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8% ± 9.6% vs. 34.6% ± 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/mL of A. sisalana crude extract [(0.81 ± 0.08) μmol/L] compared to control group [(0.41 ± 0.18) μmol/L]. Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber.
