Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan
10.1016/j.apjtb.2016.05.007
- Author:
Ismail JALIL
1
;
Muhammad ARSHAD
1
;
Zara RAFAQUE
1
;
Javid Iqbal DASTI
1
;
Fazle RAZIQ
2
;
Robina WAZIR
3
;
Sajid MALIK
4
Author Information
1. Department of Microbiology, Qauid-i-Azam University Islamabad
2. Department of Pathology, Hayatabad Medical Complex Peshawar
3. Department of Pathology, Khyber Teaching Hospital Peshawar
4. Department of Animal Sciences, Qauid-i-Azam University Islamabad
- Publication Type:Journal Article
- Keywords:
Co-infection;
HBV;
HCV;
HDV;
Risk factors
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(7):609-613
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the seroprevalence of hepatitis B virus (HBV) and hepatitis delta virus (HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of Pakistan. Methods Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel electrophoresis. Results Altogether, 946 blood samples were screened, overall percentage of HBsAg-positive patients remained 22.41% (prevalence: 224.10/1 000; CI: 0.197 5 ± 0.250 7) with the highest incidence rates among relatively younger age groups (20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1 000; CI: 0.031 8 ± 0.061 7. In HBsAg-positive patients, anti-HBc-total was detected in 86.79% while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise (62%), anorexia (66%) and fatigue (73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection (χ
