Regulation of osteoprotegerin expression by Notch signaling in human oral squamous cell carcinoma cell line
10.1016/j.apjtb.2016.06.012
- Author:
Jeeranan MANOKAWINCHOKE
1
;
Thanaphum OSATHANON
1
;
Prasit PAVASANT
1
Author Information
1. Mineralized Tissue Research Unit, Department of Anatomy, Faculty of Dentistry, Chulalongkorn University
- Publication Type:Journal Article
- Keywords:
DAPT;
Jagged1;
Notch signaling;
Oral squamous cell carcinoma;
Osteoprotegerin
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(8):692-697
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of Notch signaling on osteoprotegerin (OPG) expression in a human oral squamous cell carcinoma cell line. Methods Activation of Notch signaling was performed by seeding cells on Jagged1 immobilized surfaces. In other experiments, a γ-secretase inhibitor was added to the culture medium to inhibit intracellular Notch signaling. OPG mRNA and protein were determined by real-time PCR and ELISA, respectively. Finally, publicly available microarray database analysis was performed using connection up- or down-regulation expression analysis of microarrays software. Results Jagged1-treatment of HSC-4 cells enhanced HES1 and HEY1 mRNA expression, confirming the intracellular activation of Notch signaling. OPG mRNA and protein levels were significantly suppressed upon Jagged1 treatment. Correspondingly, HSC-4 cells treated with a γ-secretase inhibitor resulted in a significant reduction of HES1 and HEY1 mRNA levels, and a marked increase in OPG protein expression was observed. These results implied that Notch signaling regulated OPG expression in HSC-4 cells. However, Jagged1 did not alter OPG expression in another human oral squamous cell carcinoma cell line (HSC-5) or a human head and neck squamous cell carcinoma cell line (HN22). Conclusions Notch signaling regulated OPG expression in an HSC-4 cell line and this mechanism could be cell line specific.
