Antibacterial activity of Myrciaria dubia (Camu camu) against Streptococcus mutans and Streptococcus sanguinis
10.1016/j.apjtb.2016.07.008
- Author:
Rosella CAMERE-COLAROSSI
1
;
Dyanne MEDINA-FLORES
1
;
Stefany CABALLERO-GARCÍA
1
;
Frank MAYTA-TOVALINO
1
;
Juana DEL VALLE-MENDOZA
1
;
Gabriela ULLOA-URIZAR
2
;
Gabriela ULLOA-URIZAR
3
;
Juana DEL VALLE-MENDOZA
3
Author Information
1. School of Dentistry, Faculty of Health Sciences, Peruvian University of Applied Sciences-UPC
2. Research and Innovation Center of the Faculty of Health Sciences, Peruvian University of Applied Sciences-UPC
3. Molecular Biology Laboratory, Nutrition Research Institute
- Publication Type:Journal Article
- Keywords:
Antibacterial effect;
Cytotoxicity;
Medicinal plants;
Myrciaria dubia
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(9):740-744
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the antibacterial and cytotoxic effect of Myrciaria dubia (Camu camu) (M. dubia) methanol extract, against Streptococcus mutans (ATCC 25175) (S. mutans) and Streptococcus sanguinis (ATCC 10556) (S. sanguinis). Methods Two methanol extracts of M. dubia were prepared in vitro, from the seeds and pulp. Ten independent tests were prepared for each type of extract, using 0.12% chlorhexidine solution as positive control. Agar diffusion test was used by preparing wells with the experimental solutions cultivated in anaerobic conditions for 48 h at 37 °C. Meanwhile, the minimum inhibitory concentration and the cytotoxic effect over MDCK cell line was found. Results A higher antibacterial effect was observed with the methanol seed extract with an inhibitory halo of (21.36 ± 6.35) mm and (19.21 ± 5.18) mm against S. mutans and S. sanguinis, respectively. The methanol extract of the pulp had an effect of (16.20 ± 2.08) mm and (19.34 ± 2.90) mm, respectively. The minimum inhibitory concentration of the pulp extract was 62.5 μg/mL for both strains, whereas for the seed antibacterial activity was observed even at low concentrations. The CC
