Pandanus amaryllifolius leaf extract increases insulin sensitivity in high-fat diet-induced obese mice
10.1016/j.apjtb.2016.08.010
- Author:
Suphaket SAENTHAWEESUK
1
;
Jarinyaporn NAOWABOOT
2
;
Nuntiya SOMPARN
2
Author Information
1. Division of Anatomy, Department of Preclinical Science, Faculty of Medicine, Thammasat University (Rangsit Campus)
2. Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University (Rangsit Campus)
- Publication Type:Journal Article
- Keywords:
Fatty liver;
Glucose transporter 4;
Insulin sensitivity;
Obesity;
Pandanus amaryllifolius
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(10):866-871
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the effect of Pandanus amaryllifolius (P. amaryllifolius) leaf extract on the insulin resistance state in obese ICR mice. Methods Obesity was induced in mice fed with high-fat diet (45% fat) for 12 weeks. After the first six weeks on the diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day, respectively for another six weeks. At the 5th week of treatment, oral glucose tolerance test was conducted. After six weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined. The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein expression, as well as histological examination. Results Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues. Conclusions The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in alleviating the insulin dysfunction in obesity state.
