Cytotoxic, genotoxic and apoptotic effects of naringenin-oxime relative to naringenin on normal and cancer cell lines
10.1016/j.apjtb.2016.08.004
- Author:
Abdurrahim KOCYIGIT
1
;
Ismail KOYUNCU
2
;
Murat DIKILITAS
3
;
Fatemeh BAHADORI
4
;
Baki TURKKAN
5
Author Information
1. Department of Medical Biochemistry, Faculty of Medicine, Bezmialem Vakif University
2. Department of Biology, Faculty of Arts and Sciences, Harran University
3. Department of Plant Protection, Faculty of Agriculture, Harran University
4. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bezmialem Vakif University
5. Department of Chemistry, Faculty of Arts and Sciences, Harran University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Comet assay;
Cytotoxic;
Genotoxic;
Naringenin;
Naringenin-oxime;
Reactive oxygen species
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(10):872-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess and compare the cytotoxic, genotoxic, apoptotic and reactive oxygen species (ROS) generating effects of naringenin (NG) and its new derived compound naringenin-oxime (NG-Ox) on MCF-7, HT-29, PC-12 cancer and L-929 normal cell lines. Methods The cells were incubated with different doses of NG-Ox and NG (50–1 000 μmol/L) for 24 h. The cell viability was assessed based on ATP cell viability assay. Intracellular accumulation of ROS was determined using the fluorescent probes 2'7'-dichlorodihydrofluorescin diacetate. Genotoxic effects were evaluated by alkaline single cell gel electrophoresis assay (comet assay) and, the apoptotic effect was evaluated by acridine orange staining at below the IC
