Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract
10.1016/j.apjtb.2016.04.014
- Author:
Muhammad Sulaiman ZUBAIR
1
;
Syariful ANAM
1
;
Subehan LALLO
2
Author Information
1. Department of Pharmacy, Faculty of Science, Tadulako University
2. Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Hasanuddin University
- Publication Type:Journal Article
- Keywords:
Anticancer;
HeLa;
Lunacrine;
Lunasia amara;
T47D;
Total alkaloid
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(11):962-966
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization. Methods The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer cell lines, cervical cancer cells (HeLa) and breast cancer cells (T47D). Thin layer chromatography, Dragendorf, acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as well. Results The ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on HeLa and T47D cancer line cells with the IC
