Potential effect of striatin (DLBS0333), a bioactive protein fraction isolated from Channa striata for wound treatment
10.1016/j.apjtb.2016.10.008
- Author:
Puji RAHAYU
1
;
Faustine MARCELLINE
1
;
Erna SULISTYANINGRUM
1
;
Maggy Thenawidjaja SUHARTONO
1
;
Raymond Rubianto TJANDRAWINATA
1
;
Maggy Thenawidjaja SUHARTONO
2
Author Information
1. Dexa Laboratories of Biomolecular Sciences, Dexa Medica
2. Department of Food Science and Technology, Bogor Agricultural University
- Publication Type:Journal Article
- Keywords:
Albumin;
Channa striata;
DLBS0333;
Wound healing
- From:Asian Pacific Journal of Tropical Biomedicine
2016;6(12):1001-1007
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize proteins and other nutrients in striatin (DLBS0333), a bioactive protein fraction isolated from snakehead fish (Channa striata) and to investigate its wound healing activity. Methods Proteins and other constituents in striatin were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, two dimension electrophoresis, immunoblotting assay, spectroscopy and high performance liquid chromatography. The wound healing activity of striatin was studied in vitro using 3T3 fibroblast cells and in vivo using wound-induced animal model. Various parameters related to wound healing process were evaluated. Results Striatin contained four major bioactive proteins with approximate molecular weight of 8.3, 10.9, 15.4 and 16.7 kDa. In addition to proteins, striatin also contained amino acids (10 essential and 7 non essential amino acids), fatty acids (palmitic acid, oleic acid, stearic acid, linoleic acid, arachidonic acid), vitamins (vitamin A, vitamin B
