In vitro and in vivo susceptibility of Leishmania major to some medicinal plants
10.1016/j.apjtb.2016.11.008
- Author:
Fatemeh MALEKI
1
;
Mitra ZAREBAVANI
2
;
Mehdi MOHEBALI
3
;
Mohammad Saaid DAYER
4
;
Fateme HAJIALIANI
5
;
Fatemeh TABATABAIE
5
Author Information
1. Faculty of Para Medical Sciences, Iran University of Medical Sciences
2. Allied Medical Sciences, Medical Laboratory Sciences, Tehran University of Medical Sciences
3. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences
4. Department of Parasitology and Medical Entomology, Faculty of Medical Sciences, Tarbiat Modares University
5. Department of Parasitology and Mycology, Faculty of Medicine, Iran University of Medical Sciences
- Publication Type:Journal Article
- Keywords:
Glucantime;
Leishmaniasis;
Medicinal plants;
Mice;
Plant extracts
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(1):37-42
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the efficacy of some medicinal plants and systemic glucantime in a comparative manner against the causative agent of cutaneous leishmaniasis both in vitro and in BALB/c mice. Methods For in vivo testing, inbred mice were challenged with Leishmania major parasites and the resultant ulcers were treated with extract based-ointments applied topically two times per day for a period of 20 days. A group of 56 mice were randomly divided into 7 subgroups. The control group received the ointment void of extracts, whereas the reference group received glucantime only. The efficacy of treatments was evaluated by measuring ulcer diameter, parasite burden and NO production. Results Our results indicated that plant extract based-ointments were effective in reducing ulcer size and parasite burden in spleens, but their effects did not differ significantly from that of glucantime. The plant extracts tested in this study were able to increase NO production that helped parasite suppression. Conclusions Our findings indicate that the tested plant extracts are effective against Leishmania major both during in vitro and in vivo experiments, but further researches are required to recommend a potential plant extract as an alternative drug.
