Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens
- Author:
Rubén MERINO-GUZMÁN
1
;
Xochitl HERNANDEZ-VELASCO
1
;
Juan David LATORRE
2
;
Amanda Desha WOLFENDEN
2
;
Kyle Dean TEAGUE
2
;
Lucas Elzie GRAHAM
2
;
Brittany Danielle MAHAFFEY
2
;
Mikayla Francis Ann BAXTER
2
;
Billy Marshall HARGIS
2
;
Guillermo TELLEZ
2
;
Ramiro DELGADO
3
Author Information
- Publication Type:Journal Article
- Keywords: Chicken; ELISA; Immunoglobulin A; Lachrymal fluid
- From:Asian Pacific Journal of Tropical Biomedicine 2017;7(2):116-120
- CountryChina
- Language:Chinese
- Abstract: Objective To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B
