Assessment of co-segregated TLR4 genotypes among Nigerian children with asymptomatic and clinical malaria
10.1016/j.apjtb.2016.11.015
- Author:
Bamidele Abiodun IWALOKUN
1
;
Senapon Olusola IWALOKUN
2
;
Bernice Enobong UDOH
3
;
Modinat BALOGUN
4
Author Information
1. Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research
2. Olorunda Primary Health Centre
3. Department of Medical Microbiology and Parasitology, Olabisi Onabanjo University
4. Iru Primary Health Centre
- Publication Type:Journal Article
- Keywords:
Asymptomatic malaria;
Clinical malaria;
Nigeria;
TLR4 co-segregated genotypes;
TLR4 polymorphism
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(2):96-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the occurrence and pattern of Toll-like receptor 4 (TLR4) co-segregated genotypes among children with Plasmodium falciparum malaria in Nigeria. Methods In this case-control study, a total of 79 Plasmodium falciparum infected children aged 2–7 years and 105 age-matched uninfected controls of Yoruba descents in Lagos were studied. The extracted DNA samples were used for TLR4 genotyping at codons 299 (Asp > Gly) and 399 (Thr > Ile) by PCR-restriction fragment length polymorphism. Malaria infection was diagnosed by blood smear microscopy and infected children were stratified into asymptomatic, uncomplicated and severe malaria sub-groups. Malnutrition was determined by measuring the mid upper arm circumference and anemia was defined as hemoglobin < 11 g/dL. Results The proportions of children with acute malnutrition and severe anemia were 12.0% and 3.2%, respectively. Parasitemia and malnutrition were not correlated and four distinct patterns of TLR4 genotypes were found in the study population: Asp299Asp/Thr399Thr (90.2%), Asp299Gly/Thr399Thr (4.3%), Gly299Gly/Thr399Thr (3.8%) and Asp299Gly/Thr399Ile (1.6%). These genotypes did not differ significantly (P > 0.05) in frequency between infected and non-infected children. However, low and high occurrences of the TLR4 Asp299Asp/Thr399Thr and Asp299Gly/Thr399Thr genotypes were observed in the severe malaria subgroup. Conclusions This study reveals a protective role for TLR4 Asp299Gly/Thr399Ile and Asp299Asp/Thr399Thr genotypes against severe malaria in Nigerian children.
